STUDIES ON VITAL STAINING

Studies on Vital Staining

When large amounts of brilliant vital red are injected into the blood stream of dogs, the dye is gradually removed from circulation, and most of it is deposited in numerous phagocytic cells which are scattered throughout various organs and tissues. The dye occurs largely in the form of tiny red granules crowded together in the cytoplasm of these cells. If Niagara sky blue, a closely related dye...

Vital Staining of Connective Tissues

When trypan blue is injected intravenously, the animal appears blue within a few minutes, not only in its integument, but in the internal organs as well. 12 or more hours after the administration of the dye, there are innumerable granules within macrophages and other colloidopexic cells, easily demonstrable by ordinary histological technique. But the animal is blue long before such granules are...

STUDIES ON VITAL STAINING V. DOUBLE STAINING WITH BRILLIANT VITAL RED AND NIAGARA SKY BLtm. CO--nATION OF HISTOLOOICAL WITH PHYSIOLOGICAL DATA

Vital Staining of the Connective Tissues

Trypan blue injected intravenously is bound almost at once by the intercellular connective tissue elements all over the body,-by collagen, reticulin, and elastic fibers. This union of dye and tissue elements is the factor responsible for the early macroscopic blue color and is antecedent to cellular colloidopexic action. Different examples of connective tissue differ among themselves in their a...

Vital Staining of Damaged Liver Cells

Some dyes are excreted almost exclusively by the liver and the rate of their elimination from the circulating blood is used as a test of certain aspects of hepatic function in man. Rose bengal is one of these dyes,""a"' and the test in which it is used is similar to the better known procedure utilizing bromsulphalein. Clearance of rose bengal from the blood is known to be retarded in hepatic di...